What is CD47 antibody?
CD47 is an immunoglobulin that is overexpressed on the surface of many types of cancer cells. CD47 forms a signaling complex with signal-regulatory protein α (SIRPα), enabling the escape of these cancer cells from macrophage-mediated phagocytosis.
Where is CD47 found?
CD47 is a kind of protein that is found on the surface of many cells in the body. It tells circulating immune cells called macrophages not to eat these cells. The body uses the CD47 protein to protect cells that should be protected and to help dispose of cells that are aged or diseased.
How do I block CD47?
To block erythrocyte CD47, anti-CD47 B6H12 monoclonal antibody was used. The B6H12 antibody blocks the interaction between CD47 and SIRP-α. IgG was used as a control. The dose of 10 or 20 µg/mL has been found to enhance phagocytosis of tumor cells.
Who discovered CD47?
Concurrently, Eric Brown had discovered CD47 2 and suggested that it might be the TSP1 receptor. We confirmed that the 50 kDa protein was recognized by several CD47 antibodies and that the TSP1 G domain peptides augmented integrin functions such as chemotaxis and cell spreading in a CD47-dependent manner 5, 26.
Where does protein CD47 come from?
CD47 is an integral membrane protein consisting of an N-terminal extracellular IgV domain derived from the immunoglobulin superfamily followed by a presenilin domain containing five membrane-spanning segments and ending in a short variably spliced cytoplasmic sequence (Fig.
Do macrophages express CD47?
Macrophages are a key cell type in the innate immune response with CD47 being identified as a dominant macrophage checkpoint. CD47 is a “do not eat me” signal, overexpressed in myeloid malignancies that leads to tumor evasion of phagocytosis by macrophages.
What is the function of CD47?
CD47, or integrin-associated protein, is a cell surface ligand expressed in low levels by nearly all cells of the body. It plays an integral role in various immune responses as well as autoimmunity, by sending a potent “don’t eat me” signal to prevent phagocytosis.
Is CD47 an immune checkpoint?
CD47 is a novel macrophage immune checkpoint that plays a broad role in cancer immune evasion across multiple cancer types and particularly in myeloid malignancies. In addition, CD47 has been identified as an LSC marker in AML.
Is CD47 an innate immune checkpoint for tumor evasion?
Thus, CD47 is a checkpoint molecule for both innate and adaptive immunity for tumor evasion.
How effective is phagocytosis?
Phagocytes can ingest microbial pathogens, but importantly also apoptotic cells. In this way, they contribute to the clearance of billions of cells that are turned over every day. Thus phagocytosis becomes essential not only for microbial elimination, but also for tissue homeostasis.
What is another name for phagocytosis?
Phagocytes Synonyms – WordHippo Thesaurus….What is another word for phagocytes?
| dendritic cells | macrophages |
|---|---|
| neutrophils | erythrocytes |
| fibroblasts | lymphocytes |