How does enhancer trap work?

How does enhancer trap work?

Glossary:Enhancer Trap. A type of DNA construct containing a reporter gene sequence downstream of a promoter that is capable of integrating into random chromosomal locations in mouse. Integration of the enhancer trap near an enhancer allows the expression of a new mRNA encoding the reporter gene.

How many genes does it take to make a Drosophila?

The Drosophila melanogaster complete genome sequence was published in 2000. It contains 180 Mb (one third of which is centric heterochromatin) and approximately 14,000 protein-coding genes.

Why is gene tagging done?

Gene tagging Gene tagging refers to the identification of existing DNA or the introduction of new DNA that can function as a tag or label for the gene of interest.  Gene tagging is a most common method used today for selection against different biotic and abiotic stress resistances studies in crop plants.

How does gene trap work?

Gene trapping is a forward genetic approach to identify a novel gene and annotate it functionally. It simultaneously disrupts and reports expression of a gene by a transgene called a gene trap construct, which can be used as a molecular tag for rapid identification of the ‘trapped’ gene.

What is gene trap mutagenesis?

Gene-trap mutagenesis is a technique that randomly generates loss-of-function mutations and reports the expression of many mouse genes. At present, several large-scale, gene-trap screens are being carried out with various new vectors, which aim to generate a public resource of mutagenized embryonic stem (ES) cells.

What is the lifespan of Drosophila?

A typical, healthy and well-maintained outbred Drosophila population will have a median lifespan of approximately 70 days and maximum of approximately 90 days at 25 °C [11], [12].

What is the lifespan of Drosophila melanogaster?

Drosophila melanogaster is a widely used model organism that has distinct advantages in aging research, including short lifespan (mean lifespan, 2–3 months), low maintenance requirements, rich genetic resource and ease to perform genetic manipulation (1).

How are genes knocked out?

Knocking out a gene means to mutate the DNA in a way that stops the gene’s expression permanently. This is possible in all kinds of cells and organisms, using specific genetic approaches. Currently, the fastest and most direct approach to achieving specific gene knockout is to use CRISPR genome editing.