Is PBS hazardous?

Is PBS hazardous?

Material may be irritating to the mucous membranes and upper respiratory tract. May be harmful by inhalation, ingestion, or skin absorption. May cause eye, skin, or respiratory system irritation.

What is the pH of 10X PBS?

7.4
PBS – Phosphate-Buffered Saline (10X) pH 7.4, RNase-free.

Does PBS have a CAS number?

CAS# 7758-11-4 is listed on the TSCA inventory. CAS# 7778-77-0 is listed on the TSCA inventory….

CAS#	7758-11-4
Chemical Name	Potassium phosphate dibasic
Percent	0-5
EINECS/ELINCS	231-834-5

How do I make a 10X PBS buffer?

To make 1 L of 10X PBS stock solution, combine 17.8 g of Na2HPO4, 2.4 g of KH2PO4, 80 g of NaCl, 2 g of KCl, and adjust final volume to 1 L. This recipe calculator enables the accurate preparation of 10X PBS for any millimeter volume.

How do you dispose of bovine serum albumin?

Keep in suitable, closed containers for disposal. For disposal see section 13. Provide appropriate exhaust ventilation at places where dust is formed.

How do I dispose of PBS?

Place spilled material in a designated, labeled waste container. Dispose of via a licensed waste disposal contractor. For emergency responders : If specialized clothing is required to deal with the spillage, take note of any information in Section 8 on suitable and unsuitable materials.

How do you prepare 10X TBS?

To make 1 L of 10X TBS stock solution, dissolve 24 g Tris and 88 g NaCl in 900 mL of water and then adjust the pH to 7.6 and final volume to 1 L.

Is PBS an irritant?

quantities have been ingested or inhaled. may cause irritation of the nose, throat and lungs.

Is phosphate buffer toxic?

May be harmful by inhalation, ingestion, or skin absorption. May cause eye, skin, or respiratory system irritation.

How do I make my PBS sterile?

Method

1. Weigh out reagents for the selected total volume of PBS.
2. Mix with distilled water to the required volume. Adjust pH to 7.3.
3. Dispense into sterile glass bottles, sterilize by autoclaving at 121°C for 15 minutes. Use a slow exhaust.
4. When cool, tighten lids and label.

What is the pH of PBS buffer 10x?

You are here : MSDS (Material Safety Data Sheet) » Buffers/Reagents » PBS Buffer 10X, pH 7.4 bioWORLD offers PBS Buffer 10X, pH 7.4 for your research at low price. PBS buffer is of a 10X concentration, and has a pH of 7.4. PBS buffer is an isotonic and non-toxic water-based salt solution that helps maintain a constant pH.

What is PBS solution used for in cell culture?

Phosphate-buffered saline (PBS) is a balanced salt solution that is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents.

What is PBS buffer used for?

PBS buffer is an isotonic and non-toxic water-based salt solution that helps maintain a constant pH. PBS buffer 1X has many uses, including substance dilution and cell container rinsing…. read more →

What does PBS stand for?

PBS – Phosphate-Buffered Saline (10X) pH 7.4, RN… PBS – Phosphate-Buffered Saline (10X) pH 7.4, RN…