What is Stereology technique?
Stereology is the three-dimensional interpretation of two-dimensional cross sections of materials or tissues. It provides practical techniques for extracting quantitative information about a three-dimensional material from measurements made on two-dimensional planar sections of the material.
What is stereological study?
Stereology is the process of sampling and counting material using specific protocols to obtain an estimate of a quantitative parameter, such as number, length, volume, etc. Obtaining accurate estimates is a critical component of producing a study outcome with statistical validity.
What is stereological cell counting?
Stereological Cell Counting The Optical Fractionator probe is the stereological tool used to count the number of cells. Results with the optical fractionator are unbiased due to systematic random sampling of the specimen and the specific rules for counting cells within a counting frame.
How do you count cells with stereology?
Stereological Cell Counting Counting the number cells in a region of interest with stereology yields reliable quantitative data which can be used to determine if the number of cells differs between experimental and control groups. The Optical Fractionator probe is the stereological tool used to count the number of cells.
How many scientists use Stereo Investigator?
Thousands of scientists use Stereo Investigator for accurate cell counting, including Dr. Mark West, pioneer in the development of unbiased stereological methods.
How do I do stereology with Stereo Investigator?
With Stereo Investigator, stereology can be done on a microscope system from image stacks, or from whole slide images. Visit stereology.info to learn more about stereology.
How can I use stereology on 2D whole slide images?
Stereology on 2D whole slide images can be employed for counting cells with the Physical Fractionator, quantifying area and volumes with the Cavalieri probe, and for calculating the area of a small objects, such as lesions or plaques, within a larger region using the Area Fraction Fractionator.