What is E-cadherin Immunostain?
Abstract. E-cadherin immunohistochemistry is used commonly in surgical pathology practice to help distinguish lobular carcinoma in situ from ductal carcinoma in situ and invasive lobular carcinoma from invasive ductal carcinoma in histologically problematic or indeterminate cases.
What is E-cadherin expression?
E-cadherin is a calcium-dependent cell adhesion molecule that is normally expressed at adherent junctions between epithelial cells. The linkage of E-cadherin by catenins to the actin filament network of the cytoskeleton is thought to be essential for adhesion between cells within the cytoskeleton.
What does GATA3 negative mean?
The GATA3 positivity cutoff value was determined to be 4%, which was the median value of all STS cases in this study. Cases with a value > 4% were defined as GATA3-positive cases, while those with a value ≤ 4% were defined as GATA3-negative cases.
What is E-cadherin and N cadherin?
E-cadherin and N-cadherin are classical cadherins and share similar structures. They form cadherin-catenin complex where the cytoplasmic domain consists of EC repeats that bind with catenins to moderate the cytoskeletal filament containing actin.
What is the function of E-cadherin?
E-cadherin is thought to prevent the initial dissociation of epithelial cells from the original tumor mass, and loss of cell-cell adhesion and cell junctions allows cells to invade surrounding tissues and migrate to distant sites.
Do macrophages express E-cadherin?
In naive conditions, primary macrophages express very low levels of E-cadherin6.
Is E-cadherin a glycoprotein?
This family of glycoproteins is responsible for calcium-dependent mechanism of intracellular adhesion. E-cadherins are crucial in embryogenesis during several processes, including gastrulation, neurulation, and organogenesis. Furthermore, suppression of E-cadherins impairs intracellular adhesion.
What does negative for E-cadherin mean?
E-cadherin is a test that the pathologist might use to help determine if the tumor is ductal or lobular. (The cells in invasive lobular carcinomas are often negative for E-cadherin.) If your report does not mention E-cadherin, it means that this test was not needed to tell what type of cancer you have.
What is the best treatment for invasive ductal carcinoma?
For people with invasive ductal carcinoma, chemotherapy may be given before surgery to shrink the tumor or after surgery to reduce the chance of cancer returning. Chemotherapy may also be recommended as the main treatment for people with metastatic breast cancer. Radiation therapy.
What is CK7 and CK20?
CK7 is found in the glandular epithelium and epithelial tumors of lung, ovary, endometrium and breast, but is not found in GI epithelium. Conversely, CK20 is expressed principally in the normal glands and epithelial tumors of the GI tract, urothelium, and Merkel cells.
Is E-cadherin immunohistochemistry useful in classifying breast cancer cases with indeterminate histopathologic features?
Positive E-cadherin expression was also associated with tubulolobular carcinomas. Conclusions: E-cadherin immunohistochemistry is helpful in classifying breast cancer cases with indeterminate histopathologic features. E-cadherin loss is uncommon in non-lobular carcinomas but shows no correlation to currently established prognostic variables.
Is E-cadherin expression related to chemosensitivity and drug resistance in CRC cells?
The analysis of chemosensitivity and E-cadherin expression in CRC cell lines showed that the absence of E-cadherin in HCT116 cells correlated with their higher chemosensitivity, whereas HT29 cells with marked membrane E-cadherin expression were more resistant to all the drugs tested.
Is E-cadherin expression involved in the formation of lobular carcinoma?
All the lobular carcinomas were negative for E-cadherin expression, even for the lobular in situ components. These data indicate that loss of E-cadherin expression is an early event in the formation of the lobular type of breast carcinoma.
Does E-cadherin expression affect invasiveness and metastases in bladder cancer?
Conclusions: In bladder cancer altered E-cadherin expression is associated with the degree of invasiveness, lymph node metastasis and increased risk of death from bladder cancer. Furthermore, E-cadherin status is an independent predictor of disease progression in patients treated with cystectomy for transitional cell carcinoma of the bladder.