What is a Fast digest restriction enzymes?
FastDigest® restriction enzymes are an advanced line of restriction enzymes for rapid DNA digestion. All FastDigest® enzymes are 100% active in the universal FastDigest® Green buffer and are able to digest DNA in 5-15 min.
How can Restriction enzymes be removed?
Restriction enzymes are commonly inactivated by a heat treatment after digestion is complete….Inactivation and residual activity of restriction enzymes
- Heating at 60°C for 15 minutes.
- Heating at 70°C for 15 minutes.
- Ethanol precipitation.
- Phenol extraction.
Why do restriction enzymes not work?
Since a restriction enzyme may lose activity due to improper storage or handling, it is important that you always check the expiration date, verify that the enzyme has been stored at -20°C, and check the temperature of your freezer (do not allow temperatures to exceed -20°C, as multiple freeze-thaw cycles (more than 3 …
Can you digest DNA for too long?
Time-Saver qualified enzymes can cut substrate DNA in 5-15 minutes and safely digest overnight. For enzymes that are not Time-Saver Qualified, the recommended incubation time is 1 hr. In general, long incubations (several hours to overnight) are not recommended, unless digesting some gDNAs.
How do restriction enzymes cut DNA sequences?
Restriction enzymes work by shape to shape matching . It comes into contact with a DNA sequence with a shape that matches a part of the enzyme , called the restriction site. It then wraps around the DNA and causes a break in both the strands of the DNA molecule.
How much does a restriction enzyme cost?
Reaction Buffers*
| PRODUCT | Cat No | Price |
|---|---|---|
| 10X Restriction Enzyme Reaction Buffer 1 | E201 | $15.00 |
| 10X Restriction Enzyme Reaction Buffer 2 | E202 | $15.00 |
| 10X Restriction Enzyme Reaction Buffer 3 | E203 | $15.00 |
| 10X Universal Restriction Enzyme Reaction Buffer | E204 | $15.00 |
How does restriction endonuclease act on a DNA molecule?
When a restriction endonuclease recognizes a sequence, it snips through the DNA molecule by catalyzing the hydrolysis (splitting of a chemical bond by addition of a water molecule) of the bond between adjacent nucleotides.
How long is restriction enzyme digest?
How long can you run restriction digest?
For diagnostic digests, 1-2 hours is often sufficient. For digests with >1 µg of DNA used for cloning, it is recommended that you digest for at least 4 hours.
What is FastDigest restriction enzyme?
FastDigest® Restriction Enzymes by MBI Fermentas. FastDigest® restriction enzymes are an advanced line of restriction enzymes for rapid DNA digestion. All FastDigest® enzymes are 100% active in the universal FastDigest® Green buffer and are able to digest DNA in 5-15 min.
What are Fermentas FastDigest enzymes?
Thermo Scientific Fermentas FastDigest enzymes are an advanced line of restriction enzymes for rapid DNA digestion. During our 30 years of restriction enzyme research, we compiled one of the largest collections of restriction enzyme producing bacterial strains in the industry.
Why choose Thermo Scientific Fermentas FastDigest?
Our FastDigest line of restriction enzymes is ideal for use in applications that require high purity reaction components, performance reliability and simple reaction set-up. Thermo Scientific Fermentas FastDigest enzymes are an advanced line of restriction enzymes for rapid DNA digestion.
How long does it take for FastDigest enzymes to digest DNA?
All FastDigest® enzymes are 100% active in the universal FastDigest® Green buffer and are able to digest DNA in 5-15 min. This enables any combination of restriction enzymes to mork simultaneously in one reaction tube and eliminates the need for sequential digestions.