Is phenol red harmful?
Inhalation – May cause respiratory irritation. Inhalation May be harmful if inhaled. Causes respiratory tract irritation. Ingestion May be harmful if swallowed.
What is phenol red used for?
Phenol red has been used as a pH indicator dye in tissue culture media for decades (Figure 1). While its presence is not critical for maintaining cell cultures, it is often used as a quick means for researchers to check on culture stocks. Most mammalian tissues exist at a near neutral pH.
What is phenol red indicator made of?
Phenol red is 3H-2,1-Benzoxathiole 1,1-dioxide in which both of the hydrogens at position 3 have been substituted by 4-hydroxyphenyl groups. A pH indicator changing colour from yellow below pH 6.8 to bright pink above pH 8.2, it is commonly used as an indicator in cell cultures and in home swimming pool test kits.
Can you drink phenol red?
Nausea, vomiting, abdominal pain, and diarrhea are common symptoms after exposure to phenol by any route. Ingestion of phenol can also cause severe corrosive injury to the mouth, throat, esophagus, and stomach, with bleeding, perforation, scarring, or stricture formation as potential sequelae.
Is phenol a hazardous waste?
Disposal. Phenol and phenol-contaminated materials should be disposed of as hazardous waste.
How do you dispose of phenol waste?
b) Aqueous solutions or buffer containing phenol may be disposed of in shatter proof bottles using the carrier provided, via Stores as Hazardous Waste and clearly labelled as shown below. Labelling of disposal container.
What happens when phenol red turns yellow?
Phenol red is a pH indicator that is yellow at a pH below 6.8 and red at a pH above 7.4 with varying shades from yellow to red between those pH levels. If the indicator has turned yellow in the bottle this means it has become contaminated with something that has made the pH more acidic and brought the pH below 6.8.
Why is phenol red pink?
A solution of phenol red is used as a pH indicator, often in cell culture. Its color exhibits a gradual transition from yellow (λmax = 443 nm) to red (λmax = 570 nm) over the pH range 6.8 to 8.2. Above pH 8.2, phenol red turns a bright pink (fuchsia) color.
Why is phenol red yellow?
Phenol red is a pH indicator which turns yellow below a pH of 6.8 and fuchsia above a pH of 7.4. If the organism is able to utilize the carbohydrate, an acid by-product is created, which turns the media yellow. When the organism is able to use the carbohydrate, a gas by-product may be produced.
Is phenol poisonous?
Phenol is highly irritating to the skin, eyes, and mucous membranes in humans after acute (short-term) inhalation or dermal exposures. Phenol is considered to be quite toxic to humans via oral exposure.
What is phenolsulfonphthalein?
Phenolsulfonphthalein (Phenol red) is a pH indicator frequently used in cell biology laboratories. In medicine phenolsulfonphthalein is used as a test to help diagnose problems or disease of the kidneys. This test determines how well the kidneys are working. The test is based on the fact that phenol red is excreted almost entirely in the urine.
How does phenolsulfonphthalein pass out of the body?
Phenolsulfonphthalein passes out of the body almost entirely in the urine. Measuring the amount of phenolsulfonphthalein in the urine can help the doctor determine if the kidneys are working properly. How test is done: After you have emptied your bladder, phenolsulfonphthalein will be given by injection.
How is phenolsulfonphthalein test done?
How test is done: After you have emptied your bladder, phenolsulfonphthalein will be given by injection. Then you will be asked to empty your bladder into a container one or more times after the medicine is given.
How to remove phenol red from culture plates?
JOSÉ V. CASTELL, in In Vitro Methods in Pharmaceutical Research, 1997 Culture plates require extensive washing to remove phenol red present in conventional culture media which interferes in 4-nitrochatechol determination.